Peroxidase activity as a biochemical marker of insecticide use in vegetables

The insecticides use is important for crop improvement and protection, but in excessive amounts, they would induce a dysfunction of metabolic enzymatic systems plant tissues, leading to undesirable qualitative changes. In this context, we are interested peroxidase (POD), an enzyme physiology whose activity seems be conditioned by the presence soil. This work aims study impact locally used (chlorpyrifos dimethoate) on POD parsley, onion, celery garlic grown soils treated or not. extraction was performed using Tris-HCl buffer (pH 7.3); its measured substrate o-dianisidine H2O2. Our result showed that insecticide onions increased 30 % 127 341 respectively, however did not change significantly garlic. Thus, action these chemicals trivial because may alter non-target pathways, especially when doses adjusted accordingly. We found stress increase all vegetables except garlic, which tolerance insecticides. findings suggest organic farming conditions could minimize onion. add overproduction negatively affects quality reduces shelf life vegetables, thus very interesting biomarker stress.